normal human intestinal epithelial cell line hiec  (ATCC)

Journal: Heliyon

Article Title: LncRNA-HANR exacerbates malignant behaviors of cholangiocarcinoma cells through activating Notch pathway

doi: 10.1016/j.heliyon.2023.e22087

Figure Lengend Snippet: LncRNA HANR facilitates CHOL cell proliferation and migration in vitro A. RT-qPCR analysis measured HANR expression in CHOL cells (TFK-1, RBE and SSP-25, HuCC-T1) and normal human intestinal epithelial cells HIEC. Functional assays were done with CHOL cells with transfection of sh-NC, sh-HANR#1 or sh-HANR#2. B. CCK-8 assay evaluated the viability of CHOL cells at 24h, 48h and 72h. C. The proliferation of transfected CHOL cells was measured via 14-day colony formation assay. D. The migration of transfected CHOL cells was testified by measurement of wound width in wound healing assay (magnification × 100). E. CHOL cell migration was assessed by transwell assay after HANR was stably knocked down (magnification × 100). Data acquired from three independent experiments were exhibited as the mean ± SD. *P < 0.05, **P < 0.01.

Article Snippet: The above-mentioned cells and human normal intestinal epithelial cell line HIEC (ATCC, Manassas, VA, USA) were incubated in an environment with 5 % CO 2 at 37 °C.

Techniques: Migration, In Vitro, Quantitative RT-PCR, Expressing, Functional Assay, Transfection, CCK-8 Assay, Colony Assay, Wound Healing Assay, Transwell Assay, Stable Transfection